1x dnase i reaction buffer (New England Biolabs)

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New England Biolabs 1x dnase i reaction buffer
1x Dnase I Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 546 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+dnase+buffer/DNase+I+Reaction+Buffer/bio_rxiv__2025__11__27__690916-114-21-31
Average 96 stars, based on 546 article reviews

1x dnase i reaction buffer - by Bioz Stars, 2026-09

96/100 stars

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Plasmid Preparation:

Article Title: 1,6-Hexanediol, commonly used to dissolve liquid–liquid phase separated condensates, directly impairs kinase and phosphatase activities
Article Snippet: .. For DNase assay, 350 ng linearized plasmid DNA was digested with 1 μl DNase I (NEB, 2000 units/ml) diluted 1:10.000 in 1x DNase buffer (NEB) in a total reaction volume of 10 μl at 37 C. The reaction was stopped at indicated time points by addition of 1 μl 50 mM EDTA and direct heat inactivation of the DNase at 70 C for 10 min in a water bath. .. DNA was analyzed by peqGreen (peqlab) staining in a 1% agarose gel.

Isolation:

Article Title: Between computational predictions and high-throughput transcriptional profiling: in depth expression analysis of the OppB trans-membrane subunit of Escherichia coli OppABCDF oligopeptide transporter.
Article Snippet: 15 16 Bacterial oligopeptide transporters encoded by arrays of opp genes are implicated in a wide 17 variety of physiological functions including nutrient acquisition, cell-to-cell communication, 18 host-pathogen interaction.. Combining the five opp genes in one oppABCDF operon of E. coli 19 assumes a unified principle of their transcriptional regulation, which should provide a 20 comparable in scale amounts of translated products.. This, however, contradicts the 21 experimentally detected disproportion in the abundance of periplasmic OppA and the trans22 membrane subunits OppB and OppC.

Extraction:

Incubation:

Article Title: Respiratory disease in ball pythons (Python regius) experimentally infected with ball python nidovirus.
Article Snippet: .. The RNA column was washed with 400 μl RNA wash buffer and then incubated with 6 U DNase enzyme (NEB), 1x DNase buffer (NEB), and RNA wash buffer for 15 min. ..

Article Title: Macrophage extracellular traps require peptidylarginine deiminase 2 and 4 and are a source of citrullinated antigens bound by rheumatoid arthritis autoantibodies
Article Snippet: .. After incubation with stimulants for 20 hours, coverslips were washed with PBS and one coverslip from each condition (per experiment) was incubated with 20 U/ml of DNase I in 1x DNase buffer (New England Biolabs) in PBS at 37°C with 5% CO 2 for 20 minutes, while paired coverslips were incubated with DNase buffer in PBS without DNase I. ..

Article Title: Achieving single nucleotide sensitivity in direct hybridization genome imaging
Article Snippet: The in vitro transcription of VRQR-ABE7.10max mRNA reaction contains 50 ng/μL linearized VRQRABE-mRNA DNA template, ATP/CTP/GTP (5 mM each), 5 mM N1-methylpseudouridine (TriLink, N-1081-1), 4 mM CleanCap AG (TriLink, N-7113), 1 unit/μL Murine RNase Inhibitor (NEB, M0314S), 0.002 units/μL Yeast Inorganic Pyrophosphatase (NEB, M2403S) and 8 units/μL T7 RNA Polymerase (NEB, M0251S) in the transcription buffer (40 mM Tris-HCl pH 8, 20 mM spermidine, 0.02% (v/v) Triton X-100, 165 mM magnesium acetate, freshly added 10 mM DTT). .. The in vitro transcription reaction was incubated at 37 °C for 2 h, and treated with DNase I by supplying with 1X DNase buffer (NEB, B0303S) and 0.3 units/μL DNase I (M0303S) and incubating at 37 °C for 20 min. .. The reaction was purified using MegaclearTM Transcription Clean-Up Kit (Invitrogen, AM1908), and dephosphorylated using 0.25 units/μL Antarctic Phosphatase (NEB, M0289S) according to manufacturer’s protocol.

Article Title: Achieving single nucleotide sensitivity in direct hybridization genome imaging.
Article Snippet: The in vitro transcription of VRQR-ABE7.10max mRNA reaction contains 50 ng/μL linearized VRQRABE-mRNA DNA template, ATP/CTP/GTP (5mM each), 5 mM N1-methylpseudouridine (TriLink, N-1081-1), 4 mM CleanCap AG (TriLink, N-7113), 1 unit/μL Murine RNase Inhibitor (NEB, M0314S), 0.002 units/μL Yeast Inorganic Pyrophosphatase (NEB, M2403S) and 8 units/μL T7 RNA Polymerase (NEB, M0251S) in the transcription buffer (40mM TrisHCl pH 8, 20mM spermidine, 0.02% (v/v) Triton X-100, 165mM magnesium acetate, freshly added 10mM DTT). .. The in vitro transcription reaction was incubated at 37 °C for 2 h, and treated with DNase I by supplying with 1X DNase buffer (NEB, B0303S) and 0.3 units/μL DNase I (M0303S) and incubating at 37 °C for 20min. .. The reaction was purified using MegaclearTM Transcription Clean-Up Kit (Invitrogen, AM1908), and dephosphorylated using 0.25 units/μL Antarctic Phosphatase (NEB, M0289S) according to manufacturer’s protocol.

Article Title: Achieving single nucleotide sensitivity in direct hybridization genome imaging
Article Snippet: The in vitro transcription of VRQR-ABE7.10max mRNA reaction contains 50 ng/μL linearized VRQRABE-mRNA DNA template, ATP/CTP/GTP (5 mM each), 5 mM N1-methylpseudouridine (TriLink, N-1081-1), 4 mM CleanCap AG (TriLink, N-7113), 1 unit/μL Murine RNase Inhibitor (NEB, M0314S), 0.002 units/μL Yeast Inorganic Pyrophosphatase (NEB, M2403S) and 8 units/μL T7 RNA Polymerase (NEB, M0251S) in the transcription buffer (40 mM Tris-HCl pH 8, 20 mM spermidine, 0.02% (v/v) Triton X-100, 165 mM magnesium acetate, freshly added 10 mM DTT). .. The in vitro transcription reaction was incubated at 37 °C for 2 hours, and treated with DNase I by supplying with 1X DNase buffer (NEB, B0303S) and 0.3 units/μL DNase I (M0303S) and incubating at 37°C for 20 min. .. The reaction was purified using MegaclearTM Transcription Clean-Up Kit (Invitrogen, AM1908), and dephosphorylated using 0.25 units/μL Antarctic Phosphatase (NEB, M0289S) according to manufacturer’s protocol.

In Vitro:

Article Title: Achieving single nucleotide sensitivity in direct hybridization genome imaging
Article Snippet: The in vitro transcription of VRQR-ABE7.10max mRNA reaction contains 50 ng/μL linearized VRQRABE-mRNA DNA template, ATP/CTP/GTP (5 mM each), 5 mM N1-methylpseudouridine (TriLink, N-1081-1), 4 mM CleanCap AG (TriLink, N-7113), 1 unit/μL Murine RNase Inhibitor (NEB, M0314S), 0.002 units/μL Yeast Inorganic Pyrophosphatase (NEB, M2403S) and 8 units/μL T7 RNA Polymerase (NEB, M0251S) in the transcription buffer (40 mM Tris-HCl pH 8, 20 mM spermidine, 0.02% (v/v) Triton X-100, 165 mM magnesium acetate, freshly added 10 mM DTT). .. The in vitro transcription reaction was incubated at 37 °C for 2 hours, and treated with DNase I by supplying with 1X DNase buffer (NEB, B0303S) and 0.3 units/μL DNase I (M0303S) and incubating at 37°C for 20 min. .. The reaction was purified using MegaclearTM Transcription Clean-Up Kit (Invitrogen, AM1908), and dephosphorylated using 0.25 units/μL Antarctic Phosphatase (NEB, M0289S) according to manufacturer’s protocol.

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